Sequence of pGL4.26[luc2_minP

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Sequence of pGL4.26[luc2_minP_Hygro]

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Sequence of pGL4.26[luc2/minP/Hygro] : 5882 BP; 1396 A; 1637 C; 1552 G; 1297 T; 0 other;

...... KpnI(18) SacI(25),NheI(27) XhoI(33) ...... ..........

1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc 60

1 ccggattgac cggccatgga ctcgagcgat cggagctcct atagttctag accggagccg 60

.......... .......... .......... .......... .......... ..........

HindIII(65) ........ .......... .......... .......... ..........

61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg 120

61 ccggttcgaa tctgtgatct cccatatatt accttcgagc tgaaggtcga accgttaggc 120

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat 180

121 catgacaacc atttcggtgg taccttctac ggtttttgta attcttcccg ggtcgcggta 180

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg 240

181 agatgggtga gcttctgccc tggcggccgc tcgtcgacgt gtttcggtac ttcgcgatgc 240

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg 300

241 gggaccacgg gccgtggtag cggaaatggc tgcgtgtata gctccacctg taatggatgc 300

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata 360

301 ggctcatgaa gctctactcg caagccgacc gtcttcgata cttcgcgata cccgacttat 360

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg 420

361 gtttggtagc ctagcaccac acgtcgctct tatcgaacgt caagaagtac gggcacaacc 420

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc 480

421 cacgggacaa gtagccacac cgacaccggg gtcgattgct gtagatgttg ctcgcgctcg 480

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc 540

481 acgacttgtc gtacccgtag tcggtcgggt ggcagcataa gcactcgttc tttcccgacg 540

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata 600

541 ttttctagga gttgcacgtt ttcttcgatg gctagtatgt tttctagtag tagtacctat 600

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac 660

601 cgttctggct gatggtcccg aaggtttcgt acatgtggaa gcactgaagg gtaaacggtg 660

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc 720

661 ggccgaagtt gctcatgctg aagcacgggc tctcgaagct ggccctgttt tggtagcggg 720

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca 780

721 actagtactt gtcatcaccg tcatggccta acgggttccc gcatcgggat ggcgtggcgt 780

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg 840

781 ggcgaacaca ggctaagtca gtacgggcgc tggggtagaa gccgttggtc tagtaggggc 840

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg 900

841 tgtggcgata ggagtcgcac cacggtaaag tggtgccgaa gccgtacaag tggtgcgacc 900

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct 960

901 cgatgaacta gacgccgaaa gcccagcacg agtacatggc gaagctcctc ctcgataaga 960

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct 1020

961 acgcgtcgaa cgttctgata ttctaagtta gacgggacga ccacgggtgt gataaatcga 1020

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca 1080

1021 agaagcgatt ctcgtgagag tagctgttca tgctggattc gttgaacgtg ctctagcggt 1080

.......... .......... ORF 3 .......... .......... ..........

NarI(1089) .......... .......... .......... .......... ..........

1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac 1140

1081 cgccgccccg cggcgagtcg ttcctccatc cactccggca ccggtttgcg aaggtggatg 1140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg 1200

1141 gtccgtaggc ggtcccgatg ccggactgtc tttgttggtc gcggtaagac tagtgggggc 1200

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg 1260

1201 ttcccctgct gttcggaccg cgtcatccgt tccaccacgg gaagaagctc cgattccacc 1260

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg 1320

1261 acctgaacct gtggccattc tgtgacccac acttggtcgc gccgctcgac acgcaggcac 1320

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca 1380

1321 cggggtacta gtactcgccg atgcaattgt tggggctccg atgtttgcga gagtagctgt 1380

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca 1440

1381 tcctgccgac cgacgtgtcg ccgctgtagc ggatgaccct gctcctgctc gtgaagaagt 1440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac 1500

1441 agcacctggc cgacttctcg gactagttta tgttcccgat ggtccatcgg ggtcggcttg 1500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg 1560

1501 acctctcgta ggacgacgtt gtggggttgt agaagctgcg gccccagcgg ccggacgggc 1560

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga 1620

1561 tgctgctacg gccgctcgac gggcggcgtc agcagcacga ccttgtgcca ttttggtact 1620

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg 1680

1621 ggctcttcct ctagcacctg atacaccggt cggtccaatg ttggcggttc ttcgacgcgc 1680

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca 1740

1681 caccacaaca caagcacctg ctccacggat ttcctgactg gccgttcaac ctgcgggcgt 1740

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct 1800

1741 tctaggcgct ctaagagtaa ttccggttct tcccgccgtt ctagcggcac attattaaga 1800

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac 1860

1801 tctcagcccc gccggccggc gaagctcgtc tgtactattc tatgtaacta ctcaaacctg 1860

.......... .......... .......... ........ SV40_PA_term EBV_rev_pri

.......... .......... .......... .......... .......... ..........

1861 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg 1920

1861 tttggtgttg atcttacgtc acttttttta cgaaataaac actttaaaca ctacgataac 1920

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt 1980

1921 gaaataaaca ttggtaatat tcgacgttat ttgttcaatt gttgttgtta acgtaagtaa 1980

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca 2040

1981 aatacaaagt ccaagtcccc ctccacaccc tccaaaaaat ttcgttcatt ttggagatgt 2040

.......... .......... .......... .......... .......... ..........

......... BamHI(2060) ......... .......... .......... ..........

2041 aatgtggtaa aatcgataag gatccgtttg cgtattgggc gctcttccgc tgatctgcgc 2100

2041 ttacaccatt ttagctattc ctaggcaaac gcataacccg cgagaaggcg actagacgcg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttagctacct tctgaggcgg 2160

2101 tcgtggtacc ggactttatt ggagactttc tccttgaacc aatcgatgga agactccgcc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 2220

2161 tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc cgaggggtcg 2220

.......... ......... pBABE_3_pri SV40_enh SV40_pro .........

.......... .......... .......... .......... .......... ..........

2221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 2280

2221 tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac ctttcagggg 2280

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 2340

2281 tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc gttggtatca 2340

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 2400

2341 gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg taagaggcgg 2400

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 2460

2401 ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagac ggagactcga 2460

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... StuI(2493) .......... ..........

2461 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcgattc 2520

2461 taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttt tcgagctaag 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttctgacact agcgccacca tgaagaagcc cgaactcacc gctaccagcg ttgaaaaatt 2580

2521 aagactgtga tcgcggtggt acttcttcgg gcttgagtgg cgatggtcgc aactttttaa 2580

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tctcatcgag aagttcgaca gtgtgagcga cctgatgcag ttgtcggagg gcgaagagag 2640

2581 agagtagctc ttcaagctgt cacactcgct ggactacgtc aacagcctcc cgcttctctc 2640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccgagccttc agcttcgatg tcggcggacg cggctatgta ctgcgggtga atagctgcgc 2700

2641 ggctcggaag tcgaagctac agccgcctgc gccgatacat gacgcccact tatcgacgcg 2700

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgatggcttc tacaaagacc gctacgtgta ccgccacttc gccagcgctg cactacccat 2760

2701 actaccgaag atgtttctgg cgatgcacat ggcggtgaag cggtcgcgac gtgatgggta 2760

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccccgaagtg ttggacatcg gcgagttcag cgagagcctg acatactgca tcagtagacg 2820

2761 ggggcttcac aacctgtagc cgctcaagtc gctctcggac tgtatgacgt agtcatctgc 2820

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgcccaaggc gttactctcc aagacctccc cgaaacagag ctgcctgctg tgttacagcc 2880

2821 gcgggttccg caatgagagg ttctggaggg gctttgtctc gacggacgac acaatgtcgg 2880

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgtcgccgaa gctatggatg ctattgccgc cgccgacctc agtcaaacca gcggcttcgg 2940

2881 acagcggctt cgatacctac gataacggcg gcggctggag tcagtttggt cgccgaagcc 2940

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cccattcggg ccccaaggca tcggccagta cacaacctgg cgggatttca tttgcgccat 3000

2941 gggtaagccc ggggttccgt agccggtcat gtgttggacc gccctaaagt aaacgcggta 3000

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgctgatccc catgtctacc actggcagac cgtgatggac gacaccgtgt ccgccagcgt 3060

3001 acgactaggg gtacagatgg tgaccgtctg gcactacctg ctgtggcaca ggcggtcgca 3060

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agctcaagcc ctggacgaac tgatgctgtg ggccgaagac tgtcccgagg tgcgccacct 3120

3061 tcgagttcgg gacctgcttg actacgacac ccggcttctg acagggctcc acgcggtgga 3120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgtccatgcc gacttcggca gcaacaacgt cctgaccgac aacggccgca tcaccgccgt 3180

3121 gcaggtacgg ctgaagccgt cgttgttgca ggactggctg ttgccggcgt agtggcggca 3180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aatcgactgg tccgaagcta tgttcgggga cagtcagtac gaggtggcca acatcttctt 3240

3181 ttagctgacc aggcttcgat acaagcccct gtcagtcatg ctccaccggt tgtagaagaa 3240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctggcggccc tggctggctt gcatggagca gcagactcgc tacttcgagc gccggcatcc 3300

3241 gaccgccggg accgaccgaa cgtacctcgt cgtctgagcg atgaagctcg cggccgtagg 3300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgagctggcc ggcagccctc gtctgcgagc ctacatgctg cgcatcggcc tggatcagct 3360

3301 gctcgaccgg ccgtcgggag cagacgctcg gatgtacgac gcgtagccgg acctagtcga 3360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctaccagagc ctcgtggacg gcaacttcga cgatgctgcc tgggctcaag gccgctgcga 3420

3361 gatggtctcg gagcacctgc cgttgaagct gctacgacgg acccgagttc cggcgacgct 3420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgccatcgtc cgcagcgggg ccggcaccgt cggtcgcaca caaatcgctc gccggagcgc 3480

3421 acggtagcag gcgtcgcccc ggccgtggca gccagcgtgt gtttagcgag cggcctcgcg 3480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agccgtatgg accgacggct gcgtcgaggt gctggccgac agcggcaacc gccggcccag 3540

3481 tcggcatacc tggctgccga cgcagctcca cgaccggctg tcgccgttgg cggccgggtc 3540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tacacgaccg cgcgctaagg aggtaggtcg agtttaaact ctagaaccgg tcatggccgc 3600

3541 atgtgctggc gcgcgattcc tccatccagc tcaaatttga gatcttggcc agtaccggcg 3600

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgt tcgaactaga 3660

3601 ttattttata gaaataaaag taatgtagac acacaaccaa aaaacacaca agcttgatct 3660

.......... ........ synth_PA_term ........ .......... ..........

SalI(3665) .......... .......... .......... .......... ..........

3661 tgctgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg 3720

3661 acgacagctg gctacgggaa ctctcggaag ttgggtcagt cgaggaaggc cacccgcgcc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag 3780

3721 ccgtactgat agcagcggcg tgaatactga cagaagaaat agtacgttga gcatcctgtc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 3840

3781 cacggccgtc gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc agcaagccga 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 3900

3841 cgccgctcgc catagtcgag tgagtttccg ccattatgcc aataggtgtc ttagtcccct 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 3960

3901 attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg catttttccg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 4020

3961 gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt ttttagctgc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 4080

4021 gagttcagtc tccaccgctt tgggctgtcc tgatatttct atggtccgca aagggggacc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 4140

4081 ttcgagggag cacgcgagag gacaaggctg ggacggcgaa tggcctatgg acaggcggaa 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 4200

4141 agagggaagc ccttcgcacc gcgaaagagt atcgagtgcg acatccatag agtcaagcca 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... ......... ApaLI(4229) ......... .......... ..........

4201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 4260

4201 catccagcaa gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg ggctggcgac 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 4320

4261 gcggaatagg ccattgatag cagaactcag gttgggccat tctgtgctga atagcggtga 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 4380

4321 ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata catccgccac gatgtctcaa 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 4440

4381 gaacttcacc accggattga tgccgatgtg atcttcttgt cataaaccat agacgcgaga 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 4500

4441 cgacttcggt caatggaagc ctttttctca accatcgaga actaggccgt ttgtttggtg 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 4560

4501 gcgaccatcg ccaccaaaaa aacaaacgtt cgtcgtctaa tgcgcgtctt tttttcctag 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 4620

4561 agttcttcta ggaaactaga aaagatgccc cagactgcga gtcaccttgc ttttgagtgc 4620

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 4680

4621 aattccctaa aaccagtact ctaatagttt ttcctagaag tggatctagg aaaatttaat 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg 4740

4681 ttttacttca aaatttagtt agatttcata tatactcatt tgaaccagac tgtcgccggc 4740

.......... .......... .......... .......... .......... ..........

.......... PstI(4759) .......... .......... .......... ..........

4741 caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg 4800

4741 gtttacgatt tggtgacgtc accaatggtc acgaactagt cactccgtgg ctagagtcgc 4800

.......... .......... .......... .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

4801 atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt 4860

4801 tagacggata aagcaagcag gtatcaccgg actgaggggc agcacatcta gtgatgctaa 4860

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg 4920

4861 gcactcccga atggtagtcc ggggtcgcgt cgttactacg gcgctctcgg cgcaagtggc 4920

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct 4980

4921 cgggggctaa acagtcgtta cttggtcggt cgtccctccc ggctcgcttc ttcaccagga 4980

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt 5040

4981 cgatgaaaca ggcggaggta ggtcagatac tcgacgacag cactacgatc tcattcttca 5040

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc 5100

5041 agcggtcact catcaaaggc ttctcaacac cggtaacgat gaccgtagca ccatagtgcg 5100

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga 5160

5101 agcagcaagc cataccgaag caagttgaga ccaagggtcg ccagttcggc ccagtgtact 5160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt 5220

5161 agtgggtata atacttcttt acgtcagtcg aggaatcccg gaggctagca acagtcttca 5220

.......... .......... ORF 3 .......... .......... ..........

......... SacII(5231) ......... .......... .......... ..........

5221 aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc 5280

5221 ttcaaccggc gccacaacag cgagtaccat taccgtcgtg atgtgttaag agaatggcag 5280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag 5340

5281 tacggtaggc attctacgaa aaggcactgg ccgctcatga gttggttcag caaaacactc 5340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca 5400

5341 atcacatatg ccgctggttc gacgagaacg ggccgcagat atgccctgtt gtggcgcggt 5400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca 5460

5401 gtatcgtcat gaaactttca cgagtagtag cccttagcaa gaagccccgc ctttctgagt 5460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct 5520

5461 tcctagaacg gcgataactc taggtcaagc tatatcgggt gagaacgtgg gtcaactaga 5520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc 5580

5521 agtcgtagaa aatgaaagtg gtcgcaaagc cccacacgtt tttgtccgtt cgttttacgg 5580

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa 5640

5581 cgtttcttcc cttactcacg ctgtgctttt acaacctacg agtatgagca ggaaaaagtt 5640

.......... .......... ORF 3 .......... .......... ..........

.......... .......... SpeI(5666) .......... .......... ..........

5641 tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt 5700

5641 ataataactt cgtaaatagt cccaatgatc atgcagagag ttcctattca ttcattataa 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg 5760

5701 ttccatgccc tccataacct gtccggcgtt attttataga aataaaagta atgtagacac 5760

.......... .......... .......... ........ synth_PA_term ........

.......... .......... .......... .......... .......... ..........

5761 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa 5820

5761 acaaccaaaa aacacactta gctatcatga ttgtatgcga gaggtagttt tgttttgctt 5820

synth_PA_term ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct 5880

5821 tgttttgttt gatcgtttta tccgacaggg gtcacgttca cgtccacggt cttgtaaaga 5880

.......... RVpri3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ct........ .......... .......... .......... .......... .......... 5882

5881 ga........ .......... .......... .......... .......... .......... 5882

.......... .......... .......... .......... .......... ..........